we’re going to turn on UPM laboratory fermentor bioreactor unit.  we do that right over here with this green button and that will illuminate.  you’ll see a display screen it’s going to go through a bit of a self-test.  There we go it’s ready to begin okay this is what the controller looks like we’ve go to pH button ,temperature button ,dissolved oxygen level and stirrer.  we’re going to use the four that are lit up we’re not actually going to use the level to calibrate the pH probe will hit the pH button.  then the called which is short for calibrate.  we will then use this dial here to highlight not that but this one right here.  reset to default calibration values then we’re going to hit called again okay then we’re going to hit pH Measure Electrode and to actually calibrate it hit called again.  now what we’re going to do is we are going to put the pH probe and temperature probe here’s.  the temperature probe up here we’re going to put them into the pH7 buffer.  before we put them into the buffer we need to rinse it off like we normally do so it rinse it off lot drive then insert into the pH 7well it’s in the pH 7 buffer you’ll  lookover here. and if you see you can see right here it’s displaying what the actual pH value is and you can see it’s kind of changing.  you know fairly rapidly we want to let that stabilize the temperature is also reading it’s reading about twenty point four which seems to be about right. sometimes these temperature probes aren’t the best and read the wrong temperature but room temperature is kept typically around 25 degrees Celsius.  so I guess 20 might not be too far off on this winter afternoon.  we can input the temperature there by pressing start stop . you’ll see it’s going to go from zero to twenty point three give it a second there it goes twenty point three.  now let’s go back at the pH it’s still moving pretty rapidly so we’re just can’t we’re just going to wait for a couple of minutes till its stabilizes so it looks like settled on 6.58.